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Immunohistochemistry analysis of Flt-4 showing staining in the cytoplasm and membrane of paraffin-embedded mouse spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Flt-4 Rabbit Polyclonal Antibody (PA516871) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from C-terminus of human Flt-4|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16871 targets Flt-4 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with human, mouse, and rat samples.
The PA5-16871 immunogen is a synthetic peptide derived from C-terminus of human Flt-4.
Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4 (also designated VEGF-R3), putatively involved in the growth of endothelial cells, are characterized by the presence of seven immuno-globulin-like sequences in their extracellular domain. On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for eother VEGF or a VEGF-related ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
chylous ascites; FLT-4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGFR-3
AI323512; Chy; FLT-4; FLT4; FLT41; LMPH1A; PCL; VEGFR-3; VEGFR3