Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 APC and 0.5 ug of Rat IgG2a K Isotype Control PE (top) or 0.5 ug of Anti-Mouse CD135 (Flt3) PE (bottom, A16400). Cells were also stained with Fixable Viability Dye eFluor® 780 and Mouse Hematopoietic Lineage Cocktail eFluor® 450, and viable, lineage negative/low cells in the large scatter population were used for analysis.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
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