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Human T-cell leukemia cells (Jurkat) were stained with fluorescein (FITC) mouse anti-CD4 antibody and, as indicated, with Alexa Fluor®488 rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090) and Alexa Fluor® 488 goat anti-rabbit IgG antibody (Cat. No. A11008). The fluorescence values of the negative controls, in which the FITC anti-CD4 antibody was omitted, are shown (black) together with the fluorescence values of the experimental samples (green). The fluorescence values represent the average signals from the population of cells analyzed.
|Tested species reactivity||Chem|
|Host / Isotype||Rabbit / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||0.1M potassium phosphate, pH 8|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.