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Fixed and permeabilized bovine pulmonary arterial endothelial cells were labeled with the filamentous actin (F-actin) stain, fluorescein phalloidin (Cat. no. F432, right). An Alexa Fluor® 594 anti–fluorescein/Oregon Green® rabbit IgG antibody (Cat. no. A11091) converted the green fluorescence to red (left).
|Tested species reactivity||Chem|
|Host / Isotype||Rabbit / IgG|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||0.1M potassium phosphate, pH 8|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.