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Fixed and permeabilized bovine pulmonary arterial endothelial cells were labeled with the filamentous actin (F-actin) stain, fluorescein phalloidin (Cat. no. F432, right). An Alexa Fluor® 594 anti–fluorescein/Oregon Green® rabbit IgG antibody (Cat. no. A11091) converted the green fluorescence to red (left).
|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||0.1M potassium phosphate, pH 8|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Glycerin suppression of fluorescence self-quenching and improvement of heterogeneous fluoroimmunoassay sensitivity.
A-11091 was used to propose a method to suppress fluorescence self-quenching observed with highly labeled antibodies in immunoassays
|Petrou PS,Mastichiadis C,Christofidis I,Kakabakos SE||Analytical chemistry (79:647)||2007|