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Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Prod # A889) and an Alexa Fluor® 488 dye-labeled anti-rabbit secondary antibody (Prod # A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP-streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Prod # T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye-labeled anti-mouse secondary antibody (Prod # A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.
|Tested species reactivity||Chemical|
|Published species reactivity||Ferret, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||0.1M potassium phosphate, pH 8|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.
A-889 was used in immunohistochemistry to study the cholinergic circuitry between basal forebrain and auditory cortex in the ferret.
|Bajo VM,Leach ND,Cordery PM,Nodal FR,King AJ||The European journal of neuroscience (40:2922)||2014|
Amygdala projections to the lateral bed nucleus of the stria terminalis in the macaque: comparison with ventral striatal afferents.
A-889 was used in immunohistochemistry to study the signal transmission between amygdala and the lateral bed nucleus of the stria terminalis in the macaque
|deCampo DM,Fudge JL||The Journal of comparative neurology (521:3191)||2013|
|Non-human primate||Not Cited||
Specialized pathways from the primate amygdala to posterior orbitofrontal cortex.
A-889 was used in immunohistochemistry - free floating to investigate the interactions between primate amygdala and the posterior orbitofrontal cortex.
|Timbie C,Barbas H||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:8106)||2014|