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Centromeres in a human metaphase chromosome spread probed with a biotinylated human centromere–specific probe and detected by sequential labeling with fluorescein streptavidin (S869), biotinylated anti-fluorescein/Oregon Green® antibody (A982) and again with fluorescein streptavidin (S869). Labeled centromeres are clearly visible on these metaphase chromosomes and interphase cell nuclei, both of which are counterstained with propidium iodide (P1304MP, P3566, P21493).
|Tested species reactivity||Chem|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||0.1M potassium phosphate, pH 8|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.