Invitrogen

Fluorescein/Oregon Green Polyclonal Antibody, Biotin

View all (5) Fluorescein/Oregon Green antibodies

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Fluorescein/Oregon Green Antibody in Immunocytochemistry (ICC/IF) — Centromeres in a human metaphase chromosome spread probed with a biotinylated human centromere–specific probe and detected by sequential labeling with fluorescein streptavidin (S869), biotinylated anti-fluorescein/Oregon Green® antibody (A982) and again with fluorescein streptavidin (S869). Labeled centromeres are clearly visible on these metaphase chromosomes and interphase cell nuclei, both of which are counterstained with propidium iodide (P1304MP, P3566, P21493).

Product Details

A-982

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 2 publications 2 publications
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Chemical
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Fluorescein/Oregon Green
Conjugate	Biotin
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	0.1M potassium phosphate, pH 8
Contains	5mM sodium azide
Storage conditions	4° C
RRID	AB_2536213

Target Information

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates.

Tissue engineering. Part A

Zimmerlin L,Donnenberg AD,Rubin JP,Basse P,Landreneau RJ,Donnenberg VS

A-982 was used in immunohistochemistry to test if adipose-derived stem cells promote tumorigenesis of metastatic breast cancer clinical isolates

Sat Jan 01 00:00:00 EST 2011

Deformation-enhanced fluctuations in the red cell skeleton with theoretical relations to elasticity, connectivity, and spectrin unfolding.

Biophysical journal

Lee JC,Discher DE

A-982 was used to study local elasticity in the red cell's spectrin-actin network

Sat Dec 01 00:00:00 EST 2001

Detection of kappa opioid receptors on mouse thymocyte phenotypic subpopulations as assessed by flow cytometry.

The Journal of pharmacology and experimental therapeutics

Ignatowski TA,Bidlack JM

A-982 was used in flow cytometry to identify thymocyte subpopulations that express the kappa opioid receptor

Thu Jan 01 00:00:00 EST 1998

Fluorescent staining of kappa opioid receptors using naltrexamine derivatives and phycoerythrin.

Journal of immunological methods

Lawrence DM,Hutchinson I,Seyed-Mozaffari A,Archer S,Bidlack JM

A-982 was used in immunocytochemistry to optimize the method for fluorescently labeling kappa opioid receptors

Fri Feb 28 00:00:00 EST 1997

Identification of kappa opioid receptors in the immune system by indirect immunofluorescence.

Proceedings of the National Academy of Sciences of the United States of America

Lawrence DM,el-Hamouly W,Archer S,Leary JF,Bidlack JM

A-982 was used in immunocytochemistry to describe a method to visualize the kappa opioid receptor

Tue Feb 14 00:00:00 EST 1995

Bioinformatics

Protein Aliases: FITC