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Follistatin Antibody (500-P207BT-25UG) in WB
Western Blot: To detect Human Follistatin by Western Blot analysis Follistatin Polyclonal Antibody, Biotin (Product # 500-P207BT-1MG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human Follistatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P207BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Follistatin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929814
Product Specific Information
AA Sequence of recombinant protein: GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Follistatin. Anti-Human Follistatin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human Follistatin (500-P207) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Follistatin.
Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human Follistatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P207BT-1MG will be provided as 2 x 500 µg
Target Information
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activin-binding protein; Follictatin; Follistatin; follistatin isoform FST317; FS
Gene Aliases: FS; FST
UniProt ID: (Human) P19883
Entrez Gene ID: (Human) 10468
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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