|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues 2-15 of human FosB.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates (7,8) and an avian nephroblastoma virus (6). The cellular homolog, c-Fos, encodes a 62 kDa nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers, which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family (4,5). Murine FosB encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements (3). Functional homologs of c-Fos and FosB include Fra-1 and Fra-2 genes (2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.