|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues T(102) S Y S T P G L S A Y S T G G A(117) of mouse FosB.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-831 detects FosB from mouse cells and tissues.
PA1-831 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~41 kDa protein representing FosB and an ~38 kDa protein representing FosB2 in NIH 3T3 serum stimulated cells.
The PA1-831 immunogen is a synthetic peptide corresponding to residues T(102) S Y S T P G L S A Y S T G G A(117) of mouse FosB.
Reconstitute with PBS.
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2.
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the and quote;leucine-zipper and quote; motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activator protein 1 transcription factors are fundamental to v-rasHa-induced changes in gene expression in neoplastic keratinocytes.
PA1-831 was used in western blot to demonstrate the role of activator protein 1 transcription factors in the effect of v-rasHa on gene expression in neoplastic keratinocytes.
|Rutberg SE,Adams TL,Glick A,Bonovich MT,Vinson C,Yuspa SH||Cancer research (60:6332)||2000|