Proteintech

Foxp3 Monoclonal Antibody (3G3), APC

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

APC-65089-100UG

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3G3

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Wet ice

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Forkhead box protein P3; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

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Gene Aliases: Foxp3; JM2; scurfin; sf

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UniProt ID: (Mouse) Q99JB6

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Entrez Gene ID: (Mouse) 20371

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Function(s)
core promoter binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NFAT protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter B cell homeostasis cytokine production myeloid cell homeostasis CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment T cell mediated immunity tolerance induction tolerance induction to self antigen regulation of immunoglobulin production positive regulation of T cell tolerance induction regulation of T cell anergy positive regulation of T cell anergy negative regulation of chronic inflammatory response negative regulation of T cell cytokine production positive regulation of peripheral T cell tolerance induction transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of cell proliferation anatomical structure morphogenesis positive regulation of gene expression negative regulation of histone deacetylation negative regulation of NF-kappaB transcription factor activity negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production negative regulation of CREB transcription factor activity positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation positive regulation of transforming growth factor beta1 production positive regulation of immature T cell proliferation in thymus positive regulation of histone acetylation negative regulation of histone acetylation negative regulation of cytokine biosynthetic process T cell activation negative regulation of T cell proliferation negative regulation of sequence-specific DNA binding transcription factor activity regulatory T cell differentiation negative regulation of interferon-gamma biosynthetic process negative regulation of interleukin-2 biosynthetic process positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of cytokine secretion negative regulation of inflammatory response negative regulation of immune response T cell receptor signaling pathway negative regulation of T-helper 17 cell differentiation
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