|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(288) E Q E S P A S P S E S C S K(302) of human Fra2.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-832 detects fos-related antigen 2 (Fra2) from human cells.
PA1-832 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~58 kDa protein representing Fra2 from HeLa cell lysate.
The PA1-832 immunogen is a synthetic peptide corresponding to residues L(288) E Q E S P A S P S E S C S K(302) of human Fra2.
Reconstitute with PBS.
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2.
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the and quote;leucine-zipper and quote; motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased infarct size and lack of hyperphagic response after focal cerebral ischemia in peroxisome proliferator-activated receptor beta-deficient mice.
PA1-832 was used in immunohistochemistry to investigate the role of peroxisome proliferator-activated receptor beta during focal cerebral ischemia in mice
|Arsenijevic D,de Bilbao F,Plamondon J,Paradis E,Vallet P,Richard D,Langhans W,Giannakopoulos P||Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (26:433)||2006|