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Alomone Labs, LTD
Frizzled-2 (FZD2) (extracellular) Polyclonal Antibody
FIGURE: 1 / 3
Frizzled-2 (FZD2) (extracellular) Antibody (AFR-022-200UL) in WB
Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4): - 1,2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200).3,4. Anti-Frizzled-2 (FZD2) (extracellular) Antibody , preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).
Product Details
AFR-022-200UL
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)GAPPRYATLEHPFH, corresponding to amino acid residues 192-205 of rat Frizzled-2, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dfrizzled2; Drosophila polarity gene (frizzled) homologue; Drosophila polarity gene homolog 2; frizzled 2, seven transmembrane spanning receptor; frizzled family receptor 2; frizzled homolog 2; Frizzled-2; Fz-2; FzE2; rFz2
Gene Aliases: Fz-10; fz-2; Fz2; Fzd10; FZD2; fzE2; hFz2
UniProt ID: (Human) Q14332, (Rat) Q08464
Entrez Gene ID: (Human) 2535, (Rat) 64512
membranous septum morphogenesis
muscular septum morphogenesis
outflow tract morphogenesis
G-protein coupled receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
sensory perception of smell
neuron differentiation
positive regulation of cGMP metabolic process
epithelial cell differentiation
non-canonical Wnt signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of sequence-specific DNA binding transcription factor activity
hard palate development
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
inner ear receptor cell development
cochlea morphogenesis
planar cell polarity pathway involved in neural tube closure
beta-catenin destruction complex disassembly
cell activation
signal transduction
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
multicellular organism development
ventricular septum morphogenesis
cellular response to vitamin D
cellular response to growth factor stimulus
cellular response to peptide hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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