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Immunofluorescence analysis of GCSF (BVD13 3A5) was performed using 70% confluent log phase K562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with G-CSF BVD13-3A5) Rat Monoclonal Antibody (Product # AHC2034) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant human G-CSF expressed in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Granulocyte colony-stimulating factors promotes survival, proliferation and differentiation of bone marrow precursor cells. GCSF is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and terminal cellular maturation of partially differentiated hematopoietic progenitors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C17orf33; colony stimulating factor 3 (granulocyte); CSF3; Filgrastim; G-CSF; GCSF; Granulocyte colony-stimulating factor; granulocyte-colony stimulating factor; Lenograstim; Pluripoietin
C17orf33; CSF3; CSF3OS; GCSF