Western blot of forebrain lysates from wildtype and a1 knockout animals, using approximately 5-7ug of tissue per slot. Blots were incubated with Anti-GABAA-R, alpha1-Subunit, C-Terminus (Cat. No. 811-GA1C) diluted 1:1000 overnight at 4°C. The antibody labeled the ~51 kDa alpha1-subunit of the GABAA-R (Lane 1). The labeling was absent from a lysate prepared from alpha1 knockout animals (Lane 2).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the cytoplasmic loop of the alpha 1 subunit of rat GABRA1|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
This antibody is predicted to react with bovine, canine, human and non-human primate based on 100% sequence homology.
This antibody is specific for the ~51 kDa alpha1-subunit of the GABAA Receptor in Western blots of rat brain.
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AMPA and GABA(A/B) receptor subunit expression in the cortex of adult squirrel monkeys during peripheral nerve regeneration.
PA1-4658 was used in immunohistochemistry to study the immunohistochemical expression of AMPA and GABAA/B subunits in adult squirrel monkey cortex during regeneration of peripheral nerve damage
|Mowery TM,Walls SM,Garraghty PE||Brain research (1520:80)||2013|
Nerve injury-induced changes in GABA(A) and GABA(B) sub-unit expression in area 3b and cuneate nucleus of adult squirrel monkeys: further evidence of developmental recapitulation.
PA1-4658 was used in immunohistochemistry to study changes in expression of GABA-A and GABA-B subunits in the somatosensory area 3b and cuneate nucleus of primates in response to nerve injury
|Mowery TM,Sarin RM,Elliott KS,E Garraghty P||Brain research (1415:63)||2011|