Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot of forebrain lysates from wildtype and a1 knockout animals, using approximately 5-7ug of tissue per slot. Blots were incubated with Anti-GABAA-R, alpha1-Subunit, C-Terminus (Cat. No. 811-GA1C) diluted 1:1000 overnight at 4°C. The antibody labeled the ~51 kDa alpha1-subunit of the GABAA-R (Lane 1). The labeling was absent from a lysate prepared from alpha1 knockout animals (Lane 2).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the cytoplasmic loop of the alpha 1 subunit of rat GABRA1|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
This antibody is predicted to react with bovine, canine, human and non-human primate based on 100% sequence homology.
This antibody is specific for the ~51 kDa alpha1-subunit of the GABAA Receptor in Western blots of rat brain.
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AMPA and GABA(A/B) receptor subunit expression in the cortex of adult squirrel monkeys during peripheral nerve regeneration.
PA1-4658 was used in immunohistochemistry to study the immunohistochemical expression of AMPA and GABAA/B subunits in adult squirrel monkey cortex during regeneration of peripheral nerve damage
|Mowery TM,Walls SM,Garraghty PE||Brain research (1520:80)||2013|
Nerve injury-induced changes in GABA(A) and GABA(B) sub-unit expression in area 3b and cuneate nucleus of adult squirrel monkeys: further evidence of developmental recapitulation.
PA1-4658 was used in immunohistochemistry to study changes in expression of GABA-A and GABA-B subunits in the somatosensory area 3b and cuneate nucleus of primates in response to nerve injury
|Mowery TM,Sarin RM,Elliott KS,E Garraghty P||Brain research (1415:63)||2011|
C-terminus; GABA(A) receptor subunit alpha-1; GABAA alpha 1; GABAAR alpha1; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; Gamma-aminobutyric acid A Receptor alpha1; gamma-aminobutyric acid A receptor, alpha 1; Gamma-aminobutyric acid receptor subunit alpha-1