|Tested species reactivity||Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the cytoplasmic loop of the beta 1 subunit of rat GABRB1|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody is predicted to react with bovine, chicken, canine, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects a ~52 kDa protein representing GABA-A receptor beta 1 subunit from rat brain fractions.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing hyperpolarization of the membrane through the opening of a CI- channel associated with the GABA receptor (GABAa-R) subtype. GABAa-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAa-R is a multimeric subunit complex. To date six alpha, four beta, and four gamma, plus alternative splicing variants of some of these subunits have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in expression of functional GABAa-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Time- and behavioral state-dependent changes in posterior hypothalamic GABAA receptors contribute to the regulation of sleep.
OPA1-04106 was used in western blot to study the role in sleep regulation of temporal and behavioural changes in GABAA receptors in the posterior hypothalamus
|Volgin DV,Lu JW,Stettner GM,Mann GL,Ross RJ,Morrison AR,Kubin L||PloS one (9:null)||2014|
A GABAergic system in airway epithelium is essential for mucus overproduction in asthma.
OPA1-04106 was used in western blot to investigate the role of a GABAergic system in airway epithelium in mucus overproduction in asthma
|Xiang YY,Wang S,Liu M,Hirota JA,Li J,Ju W,Fan Y,Kelly MM,Ye B,Orser B,O'Byrne PM,Inman MD,Yang X,Lu WY||Nature medicine (13:862)||2007|