|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the N-terminus of the Delta subunit of rat GABA-A receptor.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1/250|
|Immunoprecipitation (IP)||10 µl/50 µg lysate|
|Western Blot (WB)||1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects a band ~52 kDa in mouse cerebellar lysates.
Gamma-aminobutyric acid (GABA) A receptors are GABA gated chloride channels, which are important mediators of neuronal inhibition in the mammalian central nervous system. Activation of the GABAA site results in increased intracellular chloride concentration and hyperpolarization of the cell. GABAA receptors are pentameric structures assembled from a family of distinct subunits. At least fourteen distinct subunits have been identified, which show distinct patterns of temporal and spatial expression. GABAA receptors may be modulated by several drug types including benzodiazepines and barbiturates, and by phosphorylation by protein kinase C (PKC) and protein kinase A (PKA). The GABA-A receptors are important targets for a variety of drugs such as benzodiazepines, barbiturates, steroids, anaesthetics and convulsants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.