Invitrogen

GABBR1 Monoclonal Antibody (S93A-49)

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GABBR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GABBR1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GABBR1 monoclonal antibody (Product # MA5-27704) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GABA-B Receptor 1 Antibody, D) Merged image. Magnification: 60x.

GABBR1 Antibody in Western Blot (WB) — Western blot analysis of GABBR1 in rat brain membrane lysate. The sample was incubated with GABBR1 monoclonal antibody (Product # MA5-27704) using a dilution of 1:1000.

GABBR1 Antibody in Immunohistochemistry (IHC) — 4 FIGURE Decrease in the synaptic localization of inhibitory synaptic markers in Tg-FDD mice. (A) Immunofluorescence for GABA A receptor (green) and GAD (red) on cortex and hippocampus. (B) Quantification of the number of merge puncta for both markers on A. (C) Double staining for GABA B receptor (green) and GAD (red). (D) Quantification of the number of merged puncta for both markers on C. p < 0.05 indicated on each graph, Mann-Whitney test, n = 9 photographs from 3 different animals per genotype. Data represented as mean +- SEM. Scale bar 10 mum.

Product Details

MA5-27704

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	S93A-49
Immunogen	Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 View immunogen
Conjugate	Unconjugated APC FITC PE PerCP
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20°C
RRID	AB_2735189

Target Information

Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vascular amyloid accumulation alters the gabaergic synapse and induces hyperactivity in a model of cerebral amyloid angiopathy.

Aging cell

Cisternas P,Taylor X,Perkins A,Maldonado O,Allman E,Cordova R,Marambio Y,Munoz B,Pennington T,Xiang S,Zhang J,Vidal R,Atwood B,Lasagna-Reeves CA

MA5-27704 was used in Immunocytochemistry to demonstrate that the disruption of GABAergic synapse organization causes this impairment and provided evidence that GABAergic synapses are impaired in patients with CAA pathology.

Thu Oct 01 00:00:00 EDT 2020

Vascular amyloid accumulation alters the gabaergic synapse and induces hyperactivity in a model of cerebral amyloid angiopathy.

Aging cell

Cisternas P,Taylor X,Perkins A,Maldonado O,Allman E,Cordova R,Marambio Y,Munoz B,Pennington T,Xiang S,Zhang J,Vidal R,Atwood B,Lasagna-Reeves CA

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: bM573K1.1.1 (gamma-aminobutyric acid (GABA) B receptor, 1a); DAAP-188P13.3; dJ271M21.1.1; dJ271M21.1.2; FLJ92613; GABA(B) receptor; GABA-B receptor 1; GABA-B receptor, R1 subunit; GABA-B-R1; Gaba-b-r1a; Gaba-b1; GABA-BR1; Gaba-br1a receptor; GABA-BR1b receptor; GABAB; GABABR1; Gababr1 receptor; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid (GABA-B) receptor, 1; Gamma-aminobutyric acid type B receptor subunit 1; gb1; GBR1; HGB1a; OTTHUMP00000109099; OTTHUMP00000214725; seven transmembrane helix receptor

Gene Aliases: bM573K1.1; GABAB1; GABABR1; GABBR1; GABBR1-3; GB1; GPRC3A

UniProt ID: (Human) Q9UBS5, (Mouse) Q9WV18, (Rat) O08620

Entrez Gene ID: (Human) 2550, (Mouse) 54393, (Rat) 81657

Molecular Function: G-protein coupled receptor