|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 465 and 558 of GAD65 (Uniprot ID#Q05329)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
PA5-21297 targets GAD65 in WB applications and shows reactivity with Human and mouse samples.
The PA5-21297 immunogen is a synthetic peptide corresponding to a region within amino acids 465 and 558 of GAD65 (Uniprot ID#Q05329).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Region-specific effects of repeated ketamine administration on the presynaptic GABAergic neurochemistry in rat brain.
PA5-21297 was used in western blot to elucidate the mechanism underlying ketamine's propsychotic-like action
|Boczek T,Lisek M,Ferenc B,Wiktorska M,Ivchevska I,Zylinska L||Neurochemistry international (91:13)||2015|
Multiple microRNAs within the 14q32 cluster target the mRNAs of major type 1 diabetes autoantigens IA-2, IA-2ß, and GAD65.
PA5-21297 was used in western blot to test if microRNAs regulate the expression of autoantigens
|Abuhatzira L,Xu H,Tahhan G,Boulougoura A,Schäffer AA,Notkins AL||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:4374)||2015|