|Tested species reactivity||Chicken, Human, Mouse, Rabbit, Reptile, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Purified, full length GAP-43 protein (both phosphorylated and unphosphorylated forms).|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Microglial involvement in neuroplastic changes following focal brain ischemia in rats.
33-5000 was used in western blot to assess the effects of post-ischemic activation of microglial cells
|Madinier A,Bertrand N,Mossiat C,Prigent-Tessier A,Beley A,Marie C,Garnier P||PloS one (4:null)||2009|
Fezf1 is required for penetration of the basal lamina by olfactory axons to promote olfactory development.
33-5000 was used in immunohistochemistry to investigate the molecular mechanisms that regulate the penetration of pioneer axons of the olfactory receptor neurons into the central nervous system basal lamina.
|Watanabe Y,Inoue K,Okuyama-Yamamoto A,Nakai N,Nakatani J,Nibu K,Sato N,Iiboshi Y,Yusa K,Kondoh G,Takeda J,Terashima T,Takumi T||The Journal of comparative neurology (515:565)||2009|
Gradual nerve elongation affects nerve cell bodies and neuro-muscular junctions.
33-5000 was used in immunohistochemistry - paraffin section to clarify the reactions of the neuro-muscular junction and nerve cell body to gradual nerve elongation
|Kazuo Ikeda KI,Masaki Matsuda MM,Daisuke Yamauchi DY,Katsuro Tomita KT,Shigenori Tanaka ST||Hand surgery : an international journal devoted to hand and upper limb surgery and related research : journal of the Asia-Pacific Federation of Societies for Surgery of the Hand (10:7)||2005|
Recovery from spinal cord injury: a new transection model in the C57Bl/6 mouse.
33-5000 was used in immunohistochemistry - paraffin section to discuss spinal cord regeneration
|Seitz A,Aglow E,Heber-Katz E||Journal of neuroscience research (67:337)||2002|
|Mouse||1:2000||Gene discovery in genetically labeled single dopaminergic neurons of the retina.||Gustincich S,Contini M,Gariboldi M,Puopolo M,Kadota K,Bono H,LeMieux J,Walsh P,Carninci P,Hayashizaki Y,Okazaki Y,Raviola E||Proceedings of the National Academy of Sciences of the United States of America (101:5069)||2004|
|Mouse||Not Cited||In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein 1 (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis.||Xu P,Yoshioka K,Yoshimura D,Tominaga Y,Nishioka T,Ito M,Nakabeppu Y||The Journal of biological chemistry (278:48422)||2003|
|Rat||Not Cited||Binding of the Ras activator son of sevenless to insulin receptor substrate-1 signaling complexes.||Baltensperger K,Kozma LM,Cherniack AD,Klarlund JK,Chawla A,Banerjee U,Czech MP||Science (New York, N.Y.) (260:1950)||1993|