|Tested species reactivity||Avian, Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Sheep, Pig, Rat, Zebrafish|
|Published species reactivity||Bovine, Human|
|Host / Isotype||Mouse / IgM, kappa|
|Immunogen||Extensively purified pig GAPDH.|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:4000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is likely to react with most mammals.
Suggested positive control: antigen standard for GAPDH (transient overexpression lysate).
GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Epigenetic dysregulation of KCa 3.1 channels induces poor prognosis in lung cancer.
MA1-16757 was used in western blot to correlate epigenomic changes with tumor aggressiveness
|Bulk E,Ay AS,Hammadi M,Ouadid-Ahidouch H,Schelhaas S,Hascher A,Rohde C,Thoennissen NH,Wiewrodt R,Schmidt E,Marra A,Hillejan L,Jacobs AH,Klein HU,Dugas M,Berdel WE,Müller-Tidow C,Schwab A||International journal of cancer (137:1306)||2015|
Host inflammatory response inhibits Escherichia coli O157:H7 adhesion to gut epithelium through augmentation of mucin expression.
MA1-16757 was used in western blot to study the role of host inflammatory response induction of MUC2 mucin secretion in reducing gut epithelial adhesion of E.coli O157:H7
|Xue Y,Zhang H,Wang H,Hu J,Du M,Zhu MJ||Infection and immunity (82:1921)||2014|
Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man.
MA1-16757 was used in immunocytochemistry to study the mechanism for rod and cone photoreceptor degeneration from rhodopsin mutations
|Cideciyan AV,Hood DC,Huang Y,Banin E,Li ZY,Stone EM,Milam AH,Jacobson SG||Proceedings of the National Academy of Sciences of the United States of America (95:7103)||1998|
Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.
MA1-16757 was used in ELISA to investigate the specificity of monoclonal anti-rhodopsin antibodies
|Hodges RS,Heaton RJ,Parker JM,Molday L,Molday RS||The Journal of biological chemistry (263:11768)||1988|