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Proteintech
GAPDH Monoclonal Antibody (1E6D9)
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Product Details
60004-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat,
Yeast,
Zebrafish
Published species
Avian,
Bovine,
Chicken,
Deer,
Dog,
Fish,
Fungi,
Hamster,
Human,
Insect,
Mammal,
Monkey,
Mouse,
Nematode,
Pig,
Rat,
Sheep,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1E6D9
Immunogen
GAPDH Fusion Protein Ag0766 (1-335 aa encoded by BC004109)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
150 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Immunogen sequence: MGKVKVGVN GFGRIGRLVT RAAFNSGKVD IVAINDPFID LNYMVYMFQY DSTHGKFHGT VKAENGKLVI NGNPITIFQE RDPSKIKWGD AGAEYVVEST GVFTTMEKAG AHLQGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIISN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGALQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTAN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTEH QVVSSDFNSD THSSTFDAGA GIALNDHFVK LISWYDNEFG YSNRVVDLMA HMASKE (1-335 aa encoded by B C004109)
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.