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GAPDH Monoclonal Antibody (1E6D9)

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GAPDH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-IG (GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IGG (red).

GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-IG ( GAPDH Antibody) at dilution of 1:50 (under 40x lens).

GAPDH Antibody in Western Blot (WB) — Arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-IG ( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GAPDH Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IGG with dilution 1:100.

GAPDH Antibody in Immunoprecipitation (IP) — IP result of anti-GAPDH (60004-1-IG for IP and Detection) with HeLa cell lysate.

Product Details

60004-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:50,000	View 1,059 publications 1,059 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 231 publications 231 publications

Product Specifications
Species Reactivity	Human, Mouse, Plant, Rat, Yeast, Zebrafish
Published species	Artificial Control, Avian, Bovine, Chicken, Deer, Dog, Fish, Fungi, Hamster, Human, Insect, Mammal, Mouse, Nematode, Pig, Rat, Sheep, Xenopus, Yeast, Zebrafish
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	1E6D9
Immunogen	GAPDH Fusion Protein Ag0766 (1-335 aa encoded by BC004109) View immunogen
Conjugate	Unconjugated HRP
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Immunogen sequence: MGKVKVGVN GFGRIGRLVT RAAFNSGKVD IVAINDPFID LNYMVYMFQY DSTHGKFHGT VKAENGKLVI NGNPITIFQE RDPSKIKWGD AGAEYVVEST GVFTTMEKAG AHLQGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIISN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGALQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTAN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTEH QVVSSDFNSD THSSTFDAGA GIALNDHFVK LISWYDNEFG YSNRVVDLMA HMASKE (1-335 aa encoded by B C004109)

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; G3PD; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908

UniProt ID: (Human) P04406, (Human) O14556, (Mouse) Q3UMT2, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86

Entrez Gene ID: (Human) 2597, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Zebrafish) 317743, (Zebrafish) 406367

Molecular Function: dehydrogenase