Immunofluorescent analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GAPDH loading control antibody (Product # MA5-15738) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast|
|Published species reactivity||Rat, Pig, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:1000 - 1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 10 publications below|
MA5-15738 detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.
MA5-15738 has been successfully used in Western blot, FACS, ELISA, IHC (P) and immunofluorescence.
MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Presynaptic stimulus-release and postsynaptic compensatory changes in mice lacking the N-type calcium channel α(1B)-subunit.
MA5-15738 was used in western blot to investigate the influence of specific voltage-dependent calcium channel deletion on synaptic function
|Petrashevskaya NN,Ishii M,D'Souza K,Koch SE,Fuller-Bicer GA,Schwartz A||Autonomic neuroscience : basic and clinical (160:9)||2011|
Up-regulation of sonic hedgehog contributes to TGF-ß1-induced epithelial to mesenchymal transition in NSCLC cells.
MA5-15738 was used in western blot to investigate the role of sonic hedgehog in epithelial to mesenchymal transition in non-small cell lung cancer
|Maitah MY,Ali S,Ahmad A,Gadgeel S,Sarkar FH||PloS one (6:null)||2011|
Induction of glucocorticoid receptor-beta expression in epithelial cells of asthmatic airways by T-helper type 17 cytokines.
MA5-15738 was used in western blot to investigate the effect of interleukin 17 on glucocorticoid receptor beta level in epithelial cells from asthmatic patients
|Vazquez-Tello A,Semlali A,Chakir J,Martin JG,Leung DY,Eidelman DH,Hamid Q||Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (40:1312)||2010|
Epithelial to mesenchymal transition is mechanistically linked with stem cell signatures in prostate cancer cells.
MA5-15738 was used in western blot to investigate the similarities between epithelial to mesenchymal transition and cancer stem-like cells in prostate cancer cells
|Kong D,Banerjee S,Ahmad A,Li Y,Wang Z,Sethi S,Sarkar FH||PloS one (5:null)||2010|
The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice.
MA5-15738 was used in western blot to investigate the role of p38 signaling in the regulation of bone development and homeostasis
|Greenblatt MB,Shim JH,Zou W,Sitara D,Schweitzer M,Hu D,Lotinun S,Sano Y,Baron R,Park JM,Arthur S,Xie M,Schneider MD,Zhai B,Gygi S,Davis R,Glimcher LH||The Journal of clinical investigation (120:2457)||2010|
The integrin co-activator Kindlin-3 is expressed and functional in a non-hematopoietic cell, the endothelial cell.
MA5-15738 was used in western blot to investigate the expression of integrin co-activator Kindlin-3 in endothelial cells
|Bialkowska K,Ma YQ,Bledzka K,Sossey-Alaoui K,Izem L,Zhang X,Malinin N,Qin J,Byzova T,Plow EF||The Journal of biological chemistry (285:18640)||2010|
Characterization of human chondrocytes exposed to simulated microgravity.
MA5-15738 was used in western blot to investigate the effect of simulated microgravity on chondrocyte characteristics
|Ulbrich C,Westphal K,Pietsch J,Winkler HD,Leder A,Bauer J,Kossmehl P,Grosse J,Schoenberger J,Infanger M,Egli M,Grimm D||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (25:551)||2010|
Application of free-flow IEF to identify protein candidates changing under microgravity conditions.
MA5-15738 was used in western blot to identify gravity-sensitive protein expression with free-flow IEF
|Pietsch J,Kussian R,Sickmann A,Bauer J,Weber G,Nissum M,Westphal K,Egli M,Grosse J,Schönberger J,Wildgruber R,Infanger M,Grimm D||Proteomics (10:904)||2010|
Heart-specific inhibition of protooncogene c-myc attenuates cold-induced cardiac hypertrophy.
MA5-15738 was used in western blot to investigate the influence of c-myc inhibition in heart on heart hypertrophy treatment
|Bello Roufai M,Li H,Sun Z||Gene therapy (14:1406)||2007|
Comparison of protective effects of trimetazidine against experimental warm ischemia of different durations: early and long-term effects in a pig kidney model.
MA5-15738 was used in western blot to investigate the effect of trimetazidine on experimental warm ischemia in a pig renal model
|Jayle C,Favreau F,Zhang K,Doucet C,Goujon JM,Hebrard W,Carretier M,Eugene M,Mauco G,Tillement JP,Hauet T||American journal of physiology. Renal physiology (292:F1082)||2007|