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Western blot analysis of GAPDH was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (Product # MA5-15738-HRP) at a dilution of 1:1000 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
|Tested species reactivity||Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast|
|Host / Isotype||Mouse / IgG1|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15738-HRP has been successfully used in Western blotting applications with yeast, bacteria, insect, human, mouse, rat, rabbit, hamster, and chicken samples.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH
BARS-38; CDABP0047; G3PD; GAPD; GAPDH; HEL-S-162eP; KNC-NDS6; OK/SW-cl.12