Western blot analysis was performed on whole cell extracts (30 ug lysate) of K-562 (Lane 1) and HEL 92.1.7 (Lane 2). The blots were probed with ABfinity™ Rabbit Oligoclonal GATA 1 Antibody (Product# 710049, 1-5 ug/ml) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate(Product # G21234,1:5000 dilution). A 50 kDa band corresponding to GATA 1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX),XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001).The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Protein corresponding to amino acids 1–200 of human GATA1|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Globin transcription factor (GATA) is a family of proteins that play a very important role in translating various signaling pathways which control the erythroid progenitor cells; to proliferate and differentiate. There are 6 transcription factors in the GATA family from GATA1 to GATA 6 of which GATA 1 plays a key role in the blood cell differentiation. To facilitate differentiation of red blood cells; GATA 1 along with binding to DNA also interacts with other regulatory proteins. GATA proteins have a high affinity to bind to DNA that show the [AT]GATA[AG] sequence. GATA 1 is found on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.