Immunofluorescent analysis of GATA2 in U2OS cells using a GATA2 recombinant rabbit oligoclonal antibody (Product # 710242) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of GATA2.
|Tested species reactivity||Human, Rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 396–409 of human GATA binding protein 2|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with equine, non-human primate, mouse, rabbit and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
GATA2 (GATA binding protein 2) belongs to the GATA family of transcription factors. GATA proteins play a very important role in signaling pathways controlling proliferation and differentiation of erythroid progenitor cells. There are 6 transcription factors in the GATA family: GATA1 to GATA6. GATA2 is a transcriptional activator regulating endothelin-1 gene expression in endothelial cells. It also appears to be involved in differentiation of other tissue types (uro-genital and nervous) during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.