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GATA2 Monoclonal Antibody (1A5)

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GATA2 Antibody in Immunocytochemistry (ICC) — Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration is 10 µg/mL.

GATA2 Antibody in Western Blot (WB) — GATA2 monoclonal antibody (M05), clone 1A5 Western Blot analysis of GATA2 expression in HeLa S3 NE (Cat # L013V3).

GATA2 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged GATA2 is approximately 0.1 ng/mL as a capture antibody.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.1 ng/mL

-

Immunofluorescence (IF)

10 µg/mL

-

Western Blot (WB)

1-5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1A5

Immunogen

GATA2 (AAH18988, 1 a.a. approximately 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: MEVAPEQPRW MAHPAVLNAQ HPDSHHPGLA HNYMEPAQLL PPDEVDVFFN HLDSQGNPYY ANPAHARARV SYSPAHARLT GGQMCRPHLL HSPGLPWLDG GK

Target Information

GATA2 (GATA binding protein 2) belongs to the GATA family of transcription factors. GATA proteins play a very important role in signaling pathways controlling proliferation and differentiation of erythroid progenitor cells. There are 6 transcription factors in the GATA family: GATA1 to GATA6. GATA2 is a transcriptional activator regulating endothelin-1 gene expression in endothelial cells. It also appears to be involved in differentiation of other tissue types (uro-genital and nervous) during embryogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Endothelial transcription factor GATA-2; FLJ45948; GATA-binding protein 2; MGC2306

Gene Aliases: DCML; GATA2; IMD21; MONOMAC; NFE1B

UniProt ID: (Human) P23769

Entrez Gene ID: (Human) 2624

Molecular Function: nuclease nucleic acid binding transcription factor zinc finger transcription factor

negative regulation of transcription from RNA polymerase II promoter urogenital system development cell fate determination neuron migration embryonic placenta development transcription, DNA-templated transcription from RNA polymerase II promoter phagocytosis positive regulation of cytosolic calcium ion concentration heart development blood coagulation organ morphogenesis tissue development regulation of primitive erythrocyte differentiation ventral spinal cord interneuron differentiation cell differentiation in hindbrain commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development pituitary gland development response to lipid somatic stem cell population maintenance regulation of histone acetylation eosinophil fate commitment inner ear morphogenesis mast cell degranulation negative regulation of fat cell differentiation positive regulation of erythrocyte differentiation negative regulation of macrophage differentiation positive regulation of megakaryocyte differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter cell maturation digestive tract development homeostasis of number of cells within a tissue positive regulation of phagocytosis positive regulation of phagocytosis, engulfment definitive hemopoiesis semicircular canal development negative regulation of fat cell proliferation GABAergic neuron differentiation negative regulation of neural precursor cell proliferation regulation of forebrain neuron differentiation

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding zinc ion binding C2H2 zinc finger domain binding