Immunofluorescent analysis of GATA3 (green) in SH-SY5Y cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a GATA3 monoclonal antibody (Product # MA1-028) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified partial recombinant human GATA3 protein expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:2000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of MA1-028 detects a prominent ~50 kDa protein in GATA3 expressing SH-SY5Y cells. The signal is enhanced in nuclear extracts due to enrichment of GATA3. This antibody has also been successfully tested in IP, IHC and Immunofluorescence applications.
MA1-028 can be used for immunofluoresence analysis of Gata3 in human ESC lines.
MA1-028 was successfully used in western blotting analysis of GATA3 in breast cancer cell lines.
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. GATA3 also regulates luminal epithelial cell differentiation in the mammary gland and is mutated in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.