This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Western blot analysis of MA1-028 detects a prominent ~50 kDa protein in GATA3 expressing SH-SY5Y cells. The signal is enhanced in nuclear extracts due to enrichment of GATA3. This antibody has also been successfully tested in IP, IHC and Immunofluorescence applications.
MA1-028 can be used for immunofluoresence analysis of Gata3 in human ESC lines.
MA1-028 was successfully used in western blotting analysis of GATA3 in breast cancer cell lines.
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Gene Aliases: Gata-3; GATA3; HDR; HDRS; jal
Molecular Function: DNA-binding transcription factor