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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1-5 µg/mL | - |
ELISA (ELISA) |
0.03 ng/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
3A4 |
Immunogen |
GATA3 (NP_001002295.1, 103 a.a. approximately 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
See Label |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Sequence of this protein is as follows: LGSHHTASPW NLSPFSKTSI HHGSPGPLSV YPPASSSSLS GGHASPHLFT FPPTPPKDVS PDPSLSTPGS AGSARQDEKE CLKYQVPLPD SMKLESSH
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GATA binding protein-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
Gene Aliases: GATA3; HDR; HDRS
UniProt ID: (Human) P23771
Entrez Gene ID: (Human) 2625
Molecular Function:
DNA-binding transcription factor
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