Immunofluorescent analysis of GATA4 using anti-GATA4 polyclonal antibody (Product # PA1-102) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with Blocker BSA (Product # 37525) for 15 minutes at room temperature and probed with a rabbit polyclonal antibody recognizing GATA4 (Product # PA1-102), at a dilution of 1:50 for 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 (green) goat-anti-rabbit secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-102 has been successfully used in Western blot, FACS, IHC (P) and immunofluorescence applications with human and mouse samples.
GATA binding factor 4 (GATA4) is a GATA family transcription regulator involved in embryonic development. In addition, members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.