OriGene

GBA3 Monoclonal Antibody (OTI5G5), TrueMAB™

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GBA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-GBA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502533)

GBA3 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3. Positive lysates LY402815 (100 µg) and LC402815 (20 µg) can be purchased separately from OriGene.

GBA3 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC211035 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody (TA502533), and then analyzed by flow cytometry.

Product Details

TA502533

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5G5
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 produced in E.coli. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.9 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides. GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytosolic beta-glucosidase; Cytosolic beta-glucosidase-like protein 1; Cytosolic GCase; Cytosolic glycosylceramidase; Glucosidase beta acid 3; glucosidase, beta, acid 3 (cytosolic); Glucosylceramidase beta 3; Klotho-related protein; KLrP

Gene Aliases: CBG; CBGL1; GBA3; GLUC; KLRP

UniProt ID: (Human) Q9H227

Entrez Gene ID: (Human) 57733

Molecular Function: glycosidase