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Invitrogen
GBL Polyclonal Antibody
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FIGURE: 1 / 5
GBL Antibody (PA5-144651) in ICC/IF
Immunocytochemistry analysis of GBL in PC-3M cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with GBL polyclonal antibody (Product # PA5-144651) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-144651
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human GBL aa 1-250.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: C2C12 cell lysate, L6 cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, 293 cell lysate, MCF-7 cell lysate, PC-3M, rat heart tissue, mouse skeletal muscle tissue.
Predicted band size: 36 kDa
Subcellular Location: Cytoplasm.
Target Information
G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G beta 6; G beta-like protein; guanine nucleotide binding protein; G beta-like protein; gable; GBL protein; mammalian lethal with SEC13 protein 8; mLST8 / mLST8; MTOR associated protein, LST8 homolog; protein GbetaL; TORC subunit gable; TORC subunit LST8; TORC subunit WAT1; transducin (beta)-like 4; unnamed protein product; WD-40 repeat protein; similar to guanine nucleotide-binding protein beta subunits
Gene Aliases: 0610033N12Rik; GbetaL; GBL; LST8; POP3; WAT1
UniProt ID: (Human) Q9BVC4, (Mouse) Q9DCJ1, (Rat) Q9Z2K5
Entrez Gene ID: (Human) 64223, (Mouse) 56716, (Rat) 64226
cellular response to DNA damage stimulus
cytoskeleton organization
negative regulation of autophagy
positive regulation of cell growth
cellular response to nutrient levels
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
TORC1 signaling
TORC2 signaling
negative regulation of apoptotic process
positive regulation of glycolytic process
positive regulation of lipid biosynthetic process
cellular response to hypoxia
cellular response to osmotic stress
positive regulation of pentose-phosphate shunt
positive regulation of actin filament polymerization
protein kinase B signaling
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of protein kinase B signaling
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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