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Invitrogen
GCLC Monoclonal Antibody (OTI1A3)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
GCLC Antibody (MA5-26346) in ICC/IF
Immunofluorescence analysis GCLC Monoclonal Antibody (OTI1A3) was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled GCLC monoclonal antibody (Product # MA5-26346) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # ... View More
Product Details
MA5-26346
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1A3
Immunogen
Full length human recombinant protein of GCLC produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2723211
Target Information
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gamma GCS-HS; Gamma-ECS; Gamma-glutamylcysteine synthetase; gamma-glutamylcysteine synthetase heavy subunit; GCS heavy chain; Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; Glutamylcysteine gamma synthetase light chain
Gene Aliases: D9Wsu168e; GCL; GCLC; GCS; Ggcs-hs; GLCL; GLCL-H; GLCLC
UniProt ID: (Human) P48506, (Rat) P19468, (Mouse) P97494
Entrez Gene ID: (Human) 2729, (Rat) 25283, (Mouse) 14629
sulfur amino acid metabolic process
cysteine metabolic process
glutamate metabolic process
glutathione biosynthetic process
response to oxidative stress
apoptotic mitochondrial changes
response to heat
response to xenobiotic stimulus
response to hormone
L-ascorbic acid metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
cell redox homeostasis
negative regulation of transcription, DNA-templated
response to arsenic-containing substance
regulation of blood vessel size
response to nitrosative stress
regulation of mitochondrial depolarization
negative regulation of extrinsic apoptotic signaling pathway
glutathione metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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