Invitrogen

GDF15 Monoclonal Antibody (CL0328)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (36) GDF15 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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GDF15 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of GDF15 in A549 cells using GDF15 Monoclonal Antibody (CL0328) (Product # MA5-31346), showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

GDF15 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of GDF15 in human prostate using a GDF15 monoclonal antibody (Product # MA5-31346). The analysis shows moderate positivity in a subset of glandular epithelium cells.

GDF15 Antibody in Western Blot (WB) — Western blot analysis of GDF15 by a GDF15 monoclonal antibody (Product # MA5-31346). Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2.

GDF15 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. RT-4 cells were transfected with, control siRNA, GDF15 siRNA probe #1, and GDF15 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-GDF15 Monoclonal Antibody (CL0328) (Product # MA5-31346) used at a dilution of 1 µg/ml. Anti-PPIB was used as a loading control. {KD}

Product Details

MA5-31346

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunocytochemistry (ICC/IF)	2-10 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	CL0328
Immunogen	Recombinant protein corresponding to Human GDF15 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2786983

Product Specific Information

Immunogen sequence: LSLAEASRAS FPGPSELHSE DSRFRELRKR YEDLLTRLRA NQSWEDSNTD LVPAPAVRIL TPEVRLGSGG HLHLRISRAA LPEGLPEASR LHRALFRLSP TASRSWDVTR PLRRQLSLAR PQAPALHLRL SPPPSQSDQL

Target Information

Bone morphogenetic proteins (e. g., BMP5, MIM 112265) are members of the transforming growth factor-beta (see TGFB1, MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GDF-15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NAG-1; NAG1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta

Gene Aliases: GDF-15; GDF15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB

UniProt ID: (Human) Q99988

Entrez Gene ID: (Human) 9518

Molecular Function: growth factor