Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of IMR-32 (Lane 1), T98G (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HEK-293 (Lane 5).The blots were probed with GDI1 Rabbit polyclonal Antibody (Product # PA3-914, 2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution).A 55 kDa band corresponding to GDI1 was observed across the cell lines tested. Apart from desired band an extra band of 50 kDa was also observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE and reg ; 4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified guanine nucleotide dissociation inhibitor, isolated from bovine brain.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-914 detects GDI from bovine samples.
PA3-914 has been successfully used in Western blot and immunofluorescence applications.
The PA3-914 immunogen is purified guanine nucleotide dissociation inhibitor, isolated from bovine brain.
GDI is a protein that is used in vesicular membrane transport for recycling of Rab GTPases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.