Immunofluorescent analysis of GFAP (green) in human diabetic Muller cells. Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature, and blocked with 5% normal goat serum for 1 hour at room temperature. Cells were probed with a GFAP monoclonal antibody (Product # MA1-35377) at a dilution of 1:25 for 1 hour at room temperature, washed with PBS, and then incubated with an Alexa Fluor 488-conjugated anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken of a fluorescent microscope at 20X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Fish, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GFAP from human brain.|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, only the 56kDa GFAP band is detected when performed on crude brain extract.
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi Lates calcarifer.
MA1-35377 was used in immunocytochemistry to establish and characterize a novel cell line infected with betanodavirus
|Chi SC,Wu YC,Cheng TM||Diseases of aquatic organisms (65:91)||2005|
Fibrous meningeal tumours with extensive non-calcifying collagenous whorls and glial fibrillary acidic protein expression: the whorling-sclerosing variant of meningioma.
MA1-35377 was used in immunohistochemistry to report on two cases of whorling-sclerosing variant of meningioma
|Haberler C,Jarius C,Lang S,Rössler K,Gruber A,Hainfellner JA,Budka H||Neuropathology and applied neurobiology (28:42)||2002|