Immunohistochemistry using NMDA Receptor Subunit 2A Polyclonal Antibody, Rabbit: Rat brain cryosections labeled with anti-NMDA receptor, subunit 2A (rat), rabbit IgG fraction (Cat. no.A6473) and detected using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. no. A11008). The tissue was also labeled with Alexa Fluor® 594 anti–glial fibrillary acidic protein antibody (Cat. no.A21295) and counterstained with TOTO®-3 iodide (Cat. no.T3604), which was pseudocolored light blue in this image.
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||protein glial fibrillary acidic protein|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.2, with 0.1% BSA|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Targeted overexpression of TGF-? in the corneal epithelium of adult transgenic mice induces changes in anterior segment morphology and activates noncanonical Wnt signaling.
A-21295 was used in immunohistochemistry to examine the effects on adult ocular surface homeostasis by TGF-alpoha overexpression
|Yuan Y,Yeh LK,Liu H,Yamanaka O,Hardie WD,Kao WW,Liu CY||Investigative ophthalmology and visual science (54:1829)||2013|