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Multicolor fluorescence analysis of mouse cerebellum cross section. Glial cells were labeled with GFAP Monoclonal Antibody, Mouse (131-17719) (Cat. no. A21282) and detected using TSA Kit ##2 (Cat. no. T20912) with the HRP conjugate of goat anti–rabbit IgG and green-fluorescent Alexa Fluor® 488 tyramide. Calbindin was labeled with an anti-calbindin primary antibody and visualized using TSA Kit ##41 (Cat. no. T30954) with the HRP conjugate of goat anti–mouse IgG and red-fluorescent Alexa Fluor® 555 tyramide. Nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. no. H1399, H3570, H21492).
|Tested species reactivity||Human|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||protein glial fibrillary acidic protein|
|Storage buffer||PBS, pH 7.2, with 0.1% BSA|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Central but not systemic administration of XPro1595 is therapeutic following moderate spinal cord injury in mice.
A-21282 was used in immunocytochemistry to elucidate the contribution of soluble TNF and transmembrane-associated TNF to the development of the lesion in the central nervous system
|Novrup HG,Bracchi-Ricard V,Ellman DG,Ricard J,Jain A,Runko E,Lyck L,Yli-Karjanmaa M,Szymkowski DE,Pearse DD,Lambertsen KL,Bethea JR||Journal of neuroinflammation (11:null)||2015|
Prokineticin receptor 2 expression identifies migrating neuroblasts and their subventricular zone transient-amplifying progenitors in adult mice.
A-21282 was used in immunocytochemistry and immunohistochemistry to study the expression of prokineticin receptor 2 and the identification of migrating neuroblasts in adult mice
|Puverel S,Nakatani H,Parras C,Soussi-Yanicostas N||The Journal of comparative neurology (512:232)||2009|
Primary cultured astrocytes from old rats are capable to activate the Nrf2 response against MPP+ toxicity after tBHQ pretreatment.
A-21282 was used in western blot to test if cortical astrocytes derived from old rats respond to tertbuthyl-hydroquinene pretreatment and promote an antioxidant rich environment
|Alarc?n-Aguilar A,Luna-L?pez A,Ventura-Gallegos JL,Lazzarini R,Galv?n-Arzate S,Gonz?lez-Puertos VY,Mor?n J,Santamar?a A,K?nigsberg M||Neurobiology of aging (35:1901)||2014|
The mood stabilizer valproate activates human FGF1 gene promoter through inhibiting HDAC and GSK-3 activities.
A-21282 was used in western blot to
|Kao CY,Hsu YC,Liu JW,Lee DC,Chung YF,Chiu IM||Journal of neurochemistry (126:4)||2013|
Curcumin-releasing mechanically adaptive intracortical implants improve the proximal neuronal density and blood-brain barrier stability.
A-21282 was used in immunohistochemistry to test if using implant softening and antioxidant release reduces neuroinflammation
|Potter KA,Jorfi M,Householder KT,Foster EJ,Weder C,Capadona JR||Acta biomaterialia (10:2209)||2014|
Distribution of leptin-sensitive cells in the postnatal and adult mouse brain.
A-21282 was used in immunohistochemistry to identify leptin-sensitive cells in the postnatal and adult rodent brain
|Caron E,Sachot C,Prevot V,Bouret SG||The Journal of comparative neurology (518:459)||2010|
glial fibrillary acidic protein, Glial fibrillary acidic protein, GFAP