The anti-GFP antibody is suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis. Anti-GFP mouse monoclonal antibody 11E5 is optimized for western analysis, allowing colorimetric detection of as little as 10 ng of GFP or GFP-fusion proteins, or chemiluminescent detection of picogram quantities.
These antibodies have been purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA before lyophilization. At the time of preparation, the products are certified to be free of unconjugated dyes. They are tested in a cytological experiment to ensure low nonspecific staining.
To prepare 0.2 mg/mL stock solutions, reconstitute the lyophilized antibodies in 0.5 mL of phosphate-buffered saline (PBS), pH 7.4. You may store these solutions for up to 3 months at 4°C with the addition of 2 mM sodium azide.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, has quickly become a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression, and a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eGFP; GFP; GFP tag; GFP2; Green Fluoresecent Protein; Turbo GFP