Invitrogen

GFP Monoclonal Antibody (GF28R), HRP

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

12 References

View all (136) GFP antibodies

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GFP Antibody (MA5-15256-HRP) in WB

Western blot was performed using Anti-GFP Monoclonal Antibody (GF28R), HRP (Product # MA5-15256-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), H3-GFP, 40 µg (Lane 3), H3-GFP, 20 µg (Lane 4), H3-GFP, 10 µg (Lane 5), p65-GFP, 40 µg (Lane 6), His-p65-YFP, 40 µg (Lane 7), H3-mCherry, 40 µg (Lane 8) and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved protei... View More

GFP Antibody in Immunocytochemistry (ICC/IF)

GFP Antibody in Immunoprecipitation (IP)

Product Image Gallery

GFP Antibody — Antibody specificity was demonstrated by detection of different targets fused to GFP tag in transiently transfected lysates tested. Relative detection of GFP tag was observed across different proteins fused with GFP in H3-GFP (Lane 3-5) and p65-GFP (Lane 6). GFP-variant, YFP is also being detected in His-p65-YFP lysate (Lane 7), using Anti-GFP Monoclonal Antibody (GF28R), HRP (Product # MA5-15256-HRP) in Western Blot. {RE}

Product Details

MA5-15256-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GF28R

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.07% Kathon

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537651

Product Specific Information

MA5-15256-HRP has been successfully used in Western blotting applications with GFP tagged samples.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Structure and mechanism of human cystine exporter cystinosin.

Cell

Guo X,Schmiege P,Assafa TE,Wang R,Xu Y,Donnelly L,Fine M,Ni X,Jiang J,Millhauser G,Feng L,Li X

MA5-15256-HRP was used in Western Blot to present structures of human cystinosin in lumen-open, cytosol-open, and cystine-bound states, which uncover the cystine recognition mechanism and capture the key conformational states of the transport cycle.

Thu Sep 29 00:00:00 UTC 2022

The role of three-tandem Pho Boxes in the control of the C-P lyase operon in a thermophilic cyanobacterium.

Environmental microbiology

Jin H,Wang Y,Fu Y,Bhaya D

Published figure using GFP monoclonal antibody (Product # MA5-15256-HRP) in Western Blot

Mon Nov 01 00:00:00 UTC 2021

Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.

Molecular cell

Iglesias N,Paulo JA,Tatarakis A,Wang X,Edwards AL,Bhanu NV,Garcia BA,Haas W,Gygi SP,Moazed D

MA5-15256-HRP was used in Western Blotting to develop a strategy for the isolation of native Schizosaccharomyces pombe heterochromatin and euchromatin fragments and analyze their composition by using quantitative mass spectrometry.

Thu Jan 02 00:00:00 UTC 2020

HdaB: a novel and conserved DnaA-related protein that targets the RIDA process to stimulate replication initiation.

Nucleic acids research

Frandi A,Collier J

Published figure using GFP monoclonal antibody (Product # MA5-15256-HRP) in Western Blot

Wed Mar 18 00:00:00 UTC 2020

Chlamydia-induced curvature of the host-cell plasma membrane is required for infection.

Proceedings of the National Academy of Sciences of the United States of America

Hänsch S,Spona D,Murra G,Köhrer K,Subtil A,Furtado AR,Lichtenthaler SF,Dislich B,Mölleken K,Hegemann JH

Published figure using GFP monoclonal antibody (Product # MA5-15256-HRP) in Immunocytochemistry

Tue Feb 04 00:00:00 UTC 2020

ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.

Nucleic acids research

Brown KC,Svendsen JM,Tucci RM,Montgomery BE,Montgomery TA

MA5-15256-HRP was used in Western Blotting to study miRNA-Argonaute interactions in C. elegans.

Wed Sep 06 00:00:00 UTC 2017

An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways.

PLoS genetics

Kamber Kaya HE,Ditzel M,Meier P,Bergmann A

Published figure using GFP Tag monoclonal antibody (Product # MA5-15256-HRP) in Immunoprecipitation

Wed Feb 01 00:00:00 UTC 2017

Nuclear export of misfolded SOD1 mediated by a normally buried NES-like sequence reduces proteotoxicity in the nucleus.

eLife

Zhong Y,Wang J,Henderson MJ,Yang P,Hagen BM,Siddique T,Vogel BE,Deng HX,Fang S

Published figure using GFP Tag monoclonal antibody (Product # MA5-15256-HRP) in Western Blot

Tue May 02 00:00:00 UTC 2017

Increased mitochondrial fusion allows the survival of older animals in diverse C. elegans longevity pathways.

Nature communications

Chaudhari SN,Kipreos ET

Published figure using GFP Tag monoclonal antibody (Product # MA5-15256-HRP) in Western Blot

Thu Aug 03 00:00:00 UTC 2017

Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.

Science signaling

Anderson DM,Makarewich CA,Anderson KM,Shelton JM,Bezprozvannaya S,Bassel-Duby R,Olson EN

MA5-15256-HRP was used in Western Blotting to identify additional members of the Sarco/endoplasmic reticulum Ca2+ ATPase-inhibitory micropeptide family, revealing a conserved mechanism for the control of intracellular Ca2+ dynamics in both muscle and nonmuscle cell types.

Tue Dec 06 00:00:00 UTC 2016

piRNAs and piRNA-Dependent siRNAs Protect Conserved and Essential C. elegans Genes from Misrouting into the RNAi Pathway.

Developmental cell

Phillips CM,Brown KC,Montgomery BE,Ruvkun G,Montgomery TA

MA5-15256-HRP was used in Western Blotting to uncover a role for piRNAs in protecting essential genes from RNA silencing.

Mon Aug 24 00:00:00 UTC 2015

c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility.

eLife

Kulasekara BR,Kamischke C,Kulasekara HD,Christen M,Wiggins PA,Miller SI

MA5-15256-HRP was used in western blot to study the role of the chemotaxis machinery in the partitioning of c-di-GMP concentrations in Pseudomonas aeruginosa

Tue Dec 17 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GFP Monoclonal Antibody (GF28R), HRP

GFP Antibody (MA5-15256-HRP) in WB

Product Details

MA5-15256-HRP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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