|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant fragment of GFP expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-15349 targets GFP in flow cytometry, immunohistochemistry, and Western blot applications and shows reactivity with Tag samples.
The MA5-15349 immunogen is purified recombinant fragment of GFP expressed in E. Coli.
MA5-15349 detects GFP which has a predicted molecular weight of approximately 27kDa.
GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo.
MA5-15349 was used in immunohistochemistry to demonstrate that the AP-1 adaptor complex and Arf1 regulate PCP protein trafficking in vivo.
|Carvajal-Gonzalez JM,Balmer S,Mendoza M,Dussert A,Collu G,Roman AC,Weber U,Ciruna B,Mlodzik M||Nature communications (6:null)||2015|