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NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312). Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.
|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Anti-Tumor Effects of Adipose Tissue Mesenchymal Stem Cell Transduced with HSV-Tk Gene on U-87-Driven Brain Tumor.
A-21312 was used in immunohistochemistry - frozen section to assess using adipose-tissue-derived mesenchymal stem cells for the treatment of glioblastoma in mice
|de Melo SM,Bittencourt S,Ferrazoli EG,da Silva CS,da Cunha FF,da Silva FH,Stilhano RS,Denapoli PM,Zanetti BF,Martin PK,Silva LM,dos Santos AA,Baptista LS,Longo BM,Han SW||PloS one (10:null)||2015|
|Not Applicable||5 µg/ml||
Translocation and insecticidal activity of Bacillus thuringiensis living inside of plants.
A-21312 was used in immunohistochemistry to study the ability of GFP-labeled B. thuringiensis to colonize plants.
|Monnerat RG,Soares CM,Capdeville G,Jones G,Martins ES,Praça L,Cordeiro BA,Braz SV,dos Santos RC,Berry C||Microbial biotechnology (2:512)||2009|
Green Fluorescence Protein