|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oxytocin receptors are expressed on dopamine and glutamate neurons in the mouse ventral tegmental area that project to nucleus accumbens and other mesolimbic targets.
A10259 was used in immunohistochemistry - free floating to characterize oxytocin receptor-expressing neurons originating within the ventral tegmental area
|Peris J,MacFadyen K,Smith JA,de Kloet AD,Wang L,Krause EG||The Journal of comparative neurology (525:1094)||2017|
Stem cell-derived endochondral cartilage stimulates bone healing by tissue transformation.
A10259 was used in immunohistochemistry - frozen section to use a murine model of a segmental tibia defect to test the utility of bone regeneration from a cartilage graft
|Bahney CS,Hu DP,Taylor AJ,Ferro F,Britz HM,Hallgrimsson B,Johnstone B,Miclau T,Marcucio RS||Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research (29:1269)||2014|