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|Tested species reactivity||Tag|
|Published species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Storage buffer||PBS, pH 7.4|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5 mL PBS, pH 7.4. Store at 2-6°C with the addition of thimerosal to a final concentration of 0.02%. For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Yeast||Not Cited||Mediator promotes CENP-a incorporation at fission yeast centromeres.||Carlsten JO,Szilagyi Z,Liu B,Lopez MD,Szászi E,Djupedal I,Nyström T,Ekwall K,Gustafsson CM,Zhu X||Molecular and cellular biology (32:4035)||2012|
Green Fluorescence Protein