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HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG<sub>2a</sub> (Cat. no. A11120) and detected with orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG (CAt. no. A21422), which colocalized with the dim GFP fluorescence. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The sample was mounted using ProLong® Gold antifade reagent (Cat. no. P36930). Some GFP fluorescence is retained in the mitochondria after fixation (top), but immunolabeling and detection greatly improve visualization (bottom).
|Tested species reactivity||Tag|
|Published species reactivity||Rat, Zebrafish, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The GFP was isolated directly from the jellyfish Aequorea victoria.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibodies in 0.5 mL PBS, pH 7.4. Store solution for up to 3 months at 4°C with the addition of 2mM sodium azide.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.
A-11120 was used in western blot to study the role of Hifs in hepatogenesis using zebrafish.
|Lin TY,Chou CF,Chung HY,Chiang CY,Li CH,Wu JL,Lin HJ,Pai TW,Hu CH,Tzou WS||PloS one (9:null)||2014|
Neurokinin-1 receptor-expressing neurons that contain serotonin and gamma-aminobutyric acid in the rat rostroventromedial medulla are involved in pain processing.
A-11120 was used in immunohistochemistry to study the role of a specific group of neurons in pain processing in the rat rostroventromedial medulla.
|Chen T,Wang XL,Qu J,Wang W,Zhang T,Yanagawa Y,Wu SX,Li YQ||The journal of pain : official journal of the American Pain Society (14:778)||2013|
Direct and retrograde transduction of nigral neurons with AAV6, 8, and 9 and intraneuronal persistence of viral particles.
A-11120 was used in immunohistochemistry to evaluate the transduction efficacy of nigral neurons with adeno-associated viral vectors
|Löw K,Aebischer P,Schneider BL||Human gene therapy (24:613)||2013|
Green Fluorescence Protein