Description: GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kappaB. GILZ has also been shown to mediate anti-inflammatory signals in macrophages and dendritic cells by interfering with NF-kappaB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing at eBioscience have observed predominantly cytoplasmic staining.
Applications Reported: This CFMKG15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.
Applications Tested: This CFMKG15 antibody has been tested by intracellular flow cytometric analysis of dexamethasone-treated mouse lymph node cells and human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For intracellular staining with CFMKG15, it is recommended to use IC Fixation Buffer (cat. 00-8222) followed by permeabilization with a 1X dilution of the 10X Permeabilization Buffer (cat. 00-8333).
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The TSC22 domain family member 3 protein (TSC22D3) is a leucine zipper protein that functions as a transcriptional regulator. The expression of TSC22D3 is stimulated by glucocorticoids and IL-10 and is thought to play a key role in the anti-inflammatory and immunosuppressive effects of these molecules. TSC22D3 can physically interact with and inhibit the activities of key inflammatory signaling mediators such NF-Kappa-B and AP-1. TSC22D3 functions as a transcriptional co-activator for various nuclear receptors and NF-Kappa-B. It has also been shown to be involved in the differentiation of mesenchymal stem cells towards osteoblasts and bone formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: delta sleep inducing peptide; delta sleep inducing peptide, immunoreactor; Delta sleep-inducing peptide immunoreactor; DSIP-immunoreactive leucine zipper protein; DSIP-immunoreactive peptide; DSIPI; GILZ; Glucocorticoid-induced leucine zipper protein; long glucocorticoid-induced leucine zipper protein; Protein DIP; Tilz3; TSC-22 related protein; TSC-22-like protein; TSC-22-related protein; TSC-22R; TSC22 domain family member 3; TSC22 domain family protein 3; TSC22 domain family, member 3; TSC22-related inducible leucine zipper 3; TSC22-related-inducible leucine zipper 3; TSC22D3
Gene Aliases: DIP; Dsip1; DSIPI; GILZ; Tilz3; TSC-22R; TSC22D3
Molecular Function: transcription factor