|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from C-terminal of human GLUT-1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||2-4 ug/ ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16793 targets GLUT-1 in IHC (P), WB, and IF applications and shows reactivity with Human and Rat samples.
The PA5-16793 immunogen is a synthetic peptide derived from C-terminal of human GLUT-1.
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.
IP-MS enrichment of GLUT1 (LFQ intensity): GLUT1 was enriched 372-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and GLUT1 antibody (Part No. PA5-16793). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy.
PA5-16793 was used in immunohistochemistry to analyze inhibition of hypoxia inducible factor 1 and acriflavine in sensitizing perihilar cholagiocarcinomas to photodynamic therapy
|Weijer R,Broekgaarden M,Krekorian M,Alles LK,van Wijk AC,Mackaaij C,Verheij J,van der Wal AC,van Gulik TM,Storm G,Heger M||Oncotarget (7:3341)||2016|
Monitoring hypoxia and vasculature during bevacizumab treatment in a murine colorectal cancer model.
PA5-16793 was used in immunohistochemistry to use a murine model of colorectal cancer to study the effects of bevacizumab therapy on tumor hypoxia and vascular density
|Heijmen L,Ter Voert EG,Punt CJ,Heerschap A,Oyen WJ,Bussink J,Sweep CG,Laverman P,Span PN,de Geus-Oei LF,Boerman OC,van Laarhoven HW||Contrast media and molecular imaging (9:237)||2014|
Kaposiform hemangioendothelioma complicated by Kasabach-Merritt phenomenon: ultrastructural observation and immunohistochemistry staining reveal the trapping of blood components.
PA5-16793 was used in immunohistochemistry to report on a case of kaposiform hemangioendothelioma complicated by Kasabach-Merritt phenomenon in an infant
|Yuan SM,Hong ZJ,Chen HN,Shen WM,Zhou XJ||Ultrastructural pathology (37:452)||2013|
Immunohistochemical expression of GLUT-1 and Ki-67 in chronic plaque psoriasis.
PA5-16793 was used in immunohistochemistry to study the expression and biological significance of Glut1 and Ki67 in chronic plaque psoriasis
|Abdou AG,Maraee AH,Eltahmoudy M,El-Aziz RA||The American Journal of dermatopathology (35:731)||2013|
18F-fluorodeoxyglucose positron emission tomography/computed tomography and the relationship between fluorodeoxyglucose uptake and the expression of hypoxia-inducible factor-1¿, glucose transporter-1 and vascular endothelial growth factor in thymic epithelial tumours.
PA5-16793 was used in immunohistochemistry to study the the clinical value of the relationships between fluorodeoxyglucose-PET/CT and HIF-1alpha, Glut1 and VEGF in thymic epithelial tumors
|Toba H,Kondo K,Sadohara Y,Otsuka H,Morimoto M,Kajiura K,Nakagawa Y,Yoshida M,Kawakami Y,Takizawa H,Kenzaki K,Sakiyama S,Bando Y,Tangoku A||European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (44:e105)||2013|
Synchrotron microbeam radiation therapy induces hypoxia in intracerebral gliosarcoma but not in the normal brain.
PA5-16793 was used in immunohistochemistry to study the differential effects of synchrotron microbeam radiation therapy on intracerebral gliosarcoma and normal brain tissue and the significance for tumor hypoxia
|Bouchet A,Lemasson B,Christen T,Potez M,Rome C,Coquery N,Le Clec'h C,Moisan A,Bräuer-Krisch E,Leduc G,Rémy C,Laissue JA,Barbier EL,Brun E,Serduc R||Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology (108:143)||2013|
Fusion of [(18)F]FDG PET with fluorescence diffuse optical tomography to improve validation of probes and tumor imaging.
PA5-16793 was used in immunohistochemistry to compare the distribution of 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) in tumors with three fluorescent probes
|Garofalakis A,Dubois A,Thézé B,Czarny B,Tavitian B,Ducongé F||Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging (15:316)||2013|
Congenital melanocytic nevus with features of hybrid schwannoma/perineurioma.
PA5-16793 was used in immunohistochemistry to report on a case of congenital melanocytic nevus
|Wang L,Wang G,Gao T||Journal of cutaneous pathology (40:497)||2013|
Transforming growth factor-ß1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
PA5-16793 was used in immunohistochemistry to study the role of TGF-beta1 in driving Schwann cell phenotypic reprogramming and fibrogenesis in response to Mycobacterium leprae
|Petito RB,Amadeu TP,Pascarelli BM,Jardim MR,Vital RT,Antunes SL,Sarno EN||Journal of neuropathology and experimental neurology (72:351)||2013|
Low-grade fibromyxoid sarcoma with prominent giant rosettes and heterotopic ossification.
PA5-16793 was used in immunohistochemistry to report on a case of low-grade fibromyxoid sarcoma displaying unusual clinicopathology
|Hisaoka M,Matsuyama A,Aoki T,Sakamoto A,Yokoyama K||Pathology, research and practice (208:557)||2012|
Differences in metabolism between adeno- and squamous cell non-small cell lung carcinomas: spatial distribution and prognostic value of GLUT1 and MCT4.
PA5-16793 was used in immunohistochemistry to study the differential immunohistochemical expression of Glut1 and MCT4 in adeno and squamous cell non-small cell lung carcinoma and the metabolic significance
|Meijer TW,Schuurbiers OC,Kaanders JH,Looijen-Salamon MG,de Geus-Oei LF,Verhagen AF,Lok J,van der Heijden HF,Rademakers SE,Span PN,Bussink J||Lung cancer (Amsterdam, Netherlands) (76:316)||2012|
FDG-PET is a good biomarker of both early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and the MEK inhibitor GDC-0973.
PA5-16793 was used in immunohistochemistry to study FDG uptake as a biomarker of early response and acquired resistance in BRAFV600 mutant melanomas treated with vemurafenib and a MEK inhibitor
|Baudy AR,Dogan T,Flores-Mercado JE,Hoeflich KP,Su F,van Bruggen N,Williams SP||EJNMMI research (2:null)||2012|
Correlation between FDG uptake by PET/CT and the expressions of glucose transporter type 1 and hexokinase II in cervical cancer.
PA5-16793 was used in immunohistochemistry to study the relationship between labelled glucose uptake and Glut1 and hexokinase II expression in lymph nodes of patients with cervical cancer
|Tong SY,Lee JM,Ki KD,Choi YJ,Seol HJ,Lee SK,Huh CY,Kim GY,Lim SJ||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (22:654)||2012|
Biological factors, tumor growth kinetics, and survival after metastasectomy for pulmonary melanoma.
PA5-16793 was used in immunohistochemistry to investigate the prognostic markers for the development of pulmonary melanoma metastases after metastasectomy
|Lee JH,Gulec SA,Kyshtoobayeva A,Sim MS,Morton DL||Annals of surgical oncology (16:2834)||2009|
The Warburg effect is genetically determined in inherited pheochromocytomas.
PA5-16793 was used in immunohistochemistry to investigate the mechanism of Warburg effect regulation in pheochromocytomas
|Favier J,Brière JJ,Burnichon N,Rivière J,Vescovo L,Benit P,Giscos-Douriez I,De Reyniès A,Bertherat J,Badoual C,Tissier F,Amar L,Libé R,Plouin PF,Jeunemaitre X,Rustin P,Gimenez-Roqueplo AP||PloS one (4:null)||2009|
Correlation between FDG uptake and glucose transporter type 1 expression in neuroendocrine tumors of the lung.
PA5-16793 was used in immunohistochemistry to investigate the association between FDG uptake and glucose transporter type 1
|Song YS,Lee WW,Chung JH,Park SY,Kim YK,Kim SE||Lung cancer (Amsterdam, Netherlands) (61:54)||2008|
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
PA5-16793 was used in immunohistochemistry - frozen section to investigate factors that regulate the retrieval of synaptic vesicles
|Pan PY,Marrs J,Ryan TA||The Journal of biological chemistry (290:22593)||2015|
Multiparametric MRI as an early biomarker of individual therapy effects during concomitant treatment of brain tumours.
PA5-16793 was used in immunohistochemistry - frozen section to use rats to assess the ability of multiparametric MRI to identify the early effects of individual treatment on brain tumors
|Lemasson B,Bouchet A,Maisin C,Christen T,Le Duc G,Rémy C,Barbier EL,Serduc R||NMR in biomedicine (28:1163)||2015|
Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models.
PA5-16793 was used in immunohistochemistry - paraffin section to assess the activity of a novel dual PI3K/mTOR inhibitor on mesothelioma
|Barbone D,Follo C,Echeverry N,Gerbaudo VH,Klabatsa A,Bueno R,Felley-Bosco E,Broaddus VC||PloS one (10:null)||2015|
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
PA5-16793 was used in western blot to study the effect of allopurinol treatment during hypoxia
|Sun Y,George J,Rocha S||PloS one (10:null)||2015|
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
PA5-16793 was used in western blot to study the regulation of HIF1-alpha mRNA stability by P-body USP52/PAN2
|Bett JS,Ibrahim AF,Garg AK,Kelly V,Pedrioli P,Rocha S,Hay RT||The Biochemical journal (451:185)||2013|
Subfield-specific neurovascular remodeling in the entorhino-hippocampal-organotypic slice culture as a response to oxygen-glucose deprivation and excitotoxic cell death.
PA5-16793 was used in western blot to study the effects of oxygen/glucose deprivation and excitotoxic neuronal death on neurovascular changes using an organotypic entorhino-hippocampal culture model
|Chip S,Nitsch C,Wellmann S,Kapfhammer JP||Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (33:508)||2013|
Sphingosine kinase 1: a new modulator of hypoxia inducible factor 1alpha during hypoxia in human cancer cells.
PA5-16793 was used in western blot to investigate the role of sphingosine kinase 1 during hypoxia in human cancer cells
|Ader I,Brizuela L,Bouquerel P,Malavaud B,Cuvillier O||Cancer research (68:8635)||2008|
Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling.
PA5-16793 was used in immunohistochemistry - paraffin section to evaluate the role of Gpr124 in CNS angiogenesis and blood-brain barrier integrity
|Zhou Y,Nathans J||Developmental cell (31:248)||2014|
Targeting Glut1-overexpressing MDA-MB-231 cells with 2-deoxy-D-g1ucose modified SPIOs.
PA5-16793 was used in immunocytochemistry to study the ability of 2-deoxy-D-glucose-modified SPIOs to target Glut1-overexpressing MDA-MB-231 tumor cells
|Shan XH,Hu H,Xiong F,Gu N,Geng XD,Zhu W,Lin J,Wang YF||European journal of radiology (81:95)||2012|
Human monocyte-derived hemangioma-like endothelial cells: evidence from an in vitro study.
PA5-16793 was used in immunocytochemistry to investigate the pathogenesis of hemangioma
|Jia J,Huang X,Zhang WF,Zhao YF||Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (17:212)||2008|