NeoBiotechnologies

GLUT-1 Monoclonal Antibody (GLUT1, 2475)

View all (32) GLUT1 antibodies

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Protocols

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GLUT-1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of K562 cells using GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot..

GLUT-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human Bladder carcinoma using GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1).

GLUT-1 Antibody in Western Blot (WB) — Western Blot analysis of Human A431 cell lysate using GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1).

GLUT-1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of K562 cells using GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

GLUT-1 Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÔøΩs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÔøΩs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

GLUT-1 Antibody — SDS-PAGE Analyis Purified GLUT-1 Monoclonal Antibody (Product # 6513-MSM4-P1). Confirmation of Purity and Integrity of Antibody.

Product Details

6513-MSM4-P1

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	1-2 µg/mL	-
Flow Cytometry (Flow)	1-2 µg/10^6 cells	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b, kappa
Class	Monoclonal
Type	Antibody
Clone	GLUT1, 2475
Immunogen	Recombinant fragment of human GLUT1 protein (around aa 203-305) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	200 µg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	4° C

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2, facilitated glucose transporter member 1

Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN; SLC2A1

UniProt ID: (Human) P11166

Entrez Gene ID: (Human) 6513

Function(s)

lactose biosynthetic process protein complex assembly response to osmotic stress glucose transport L-ascorbic acid metabolic process cellular response to glucose starvation xenobiotic transport regulation of insulin secretion dehydroascorbic acid transport glucose transmembrane transport

Process(es)

glucose transmembrane transporter activity protein binding kinase binding dehydroascorbic acid transporter activity identical protein binding xenobiotic transporter activity protein self-association D-glucose transmembrane transporter activity

GLUT-1 Monoclonal Antibody (GLUT1, 2475)

Product Details

6513-MSM4-P1

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Peptide Array (Array)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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