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Invitrogen
GLUT1 Monoclonal Antibody (10C10)
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FIGURE: 1 / 6
GLUT1 Antibody (MA5-49148) in ICC/IF
Immunocytochemistry analysis of GLUT1 in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in GLUT1 Monoclonal antibody (Product # MA5-49148) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10C10
Immunogen
E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.
Positive Control - WB: human placenta tissue, human placenta tissue. IHC: human placenta tissue, human renal cancer tissue. ICC/IF: SiHa cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Glucose transporter 1 (GLUT1), encoded by the SLC2A1 gene, is a facilitative glucose transporter that plays a pivotal role in the uptake of glucose across cell membranes, particularly in tissues with high-energy demands such as the brain, erythrocytes, and endothelial cells of the blood-brain barrier. GLUT1 is ubiquitously expressed and functions through a passive diffusion mechanism, allowing glucose to move down its concentration gradient. Its involvement in maintaining glucose homeostasis is crucial for cellular metabolism and energy production. Mutations in SLC2A1 can lead to GLUT1 deficiency syndrome, characterized by seizures, developmental delay, and movement disorders due to impaired glucose transport into the brain. GLUT1 also plays a significant role in cancer biology, where its overexpression is commonly observed in various tumors, facilitating the enhanced glucose uptake required for rapid tumor cell proliferation. This characteristic makes GLUT1 a potential target for cancer diagnostics and therapeutics. Additionally, GLUT1's regulation is influenced by factors such as hypoxia, insulin, and cellular stress, highlighting its adaptive role in different physiological and pathological states.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); dystonia gene 18; dystonia gene 9; glucose transporter glut1; glucose transporter glycoprotein; glucose transporter type 1, erythrocyte/brain; hepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; unnamed protein product
Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN
UniProt ID: (Human) P11166
Entrez Gene ID: (Human) 6513
response to hypoxia
central nervous system development
female pregnancy
fucose transmembrane transport
plasma membrane long-chain fatty acid transport
L-ascorbic acid metabolic process
cerebral cortex development
response to insulin
xenobiotic transport
photoreceptor cell maintenance
transmembrane transport
protein-containing complex assembly
dehydroascorbic acid transport
cellular response to mechanical stimulus
cellular hyperosmotic response
glucose import across plasma membrane
transport across blood-brain barrier
response to Thyroglobulin triiodothyronine
glucose transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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