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Invitrogen
GNAI1/GNAI2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
GNAI1/GNAI2 Antibody (PA1-1000) in ICC/IF
Immunofluorescence analysis of G Protein alpha Inhibitor 1 & 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GNAI1/GNAI2 Rabbit Polyclonal Antibody (Product # PA1-1000) at 2 µg/mL in 0.1% BSA and i... View More
Product Details
PA1-1000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2232440
Product Specific Information
PA1-1000 detects G protein alpha inhibitor 1/2 from human, rat, and mouse tissues and cells.
PA1-1000 has been successfully used in Immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing G protein alpha inhibitor 1/2 from HeLa cell lysate.
The PA1-1000 immunogen is a synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2. This sequence is completely conserved in human, rat, guinea pig, hamster, bovine, hydra, octopus, Xenopus, chicken, canine, spiny dogfish, great pond snail, starfish, and American lobster G protein alpha inhibitor 1/2. PA1-1000 immunizing peptide (Cat. # PEP-129) is available for use in neutralization and control procedures.
Target Information
The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adenylate cyclase-inhibiting G alpha protein; G alpha inhibitory type 2 (a); G alpha inhibitory type 2 (b); Gi1 protein alpha subunit; GTP-binding protein (G-alpha-i2); GTP-binding regulatory protein Gi alpha-2 chain; guanine nucleotide binding protein (G protein), alpha inhibiting 1; guanine nucleotide binding protein (G protein), alpha inhibiting 2; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2; guanine nucleotide binding protein, alpha inhibiting 1; guanine nucleotide binding protein, alpha inhibiting 2; Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(i) subunit alpha-2; guanine nucleotide-binding protein G(i), alpha-1 subunit; guanine nucleotide-binding protein G(i), alpha-2 subunit
Gene Aliases: AU046200; BPGTPB; C76432; Galphai2; Gi; Gia; Gialpha1; GIP; Gnai-1; Gnai-2; GNAI1; GNAI2; GNAI2B; H_LUCA15.1; H_LUCA16.1
UniProt ID: (Human) P63096, (Human) P04899, (Rat) P10824, (Mouse) B2RSH2, (Rat) P04897, (Mouse) P08752
Entrez Gene ID: (Human) 2770, (Human) 2771, (Rat) 25686, (Mouse) 14677, (Rat) 81664, (Mouse) 14678
magnesium ion binding
G-protein coupled receptor binding
GTPase activity
signal transducer activity
protein binding
GTP binding
GDP binding
G-protein beta/gamma-subunit complex binding
G-protein coupled serotonin receptor binding
GTPase activating protein binding
metal ion binding
nucleotide binding
guanyl nucleotide binding
heterotrimeric G-protein
protein folding
cell cycle
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
response to peptide hormone
regulation of cAMP-mediated signaling
negative regulation of synaptic transmission
cell division
cellular response to forskolin
activation of MAPKK activity
adenosine receptor signaling pathway
signal transduction
negative regulation of adenylate cyclase activity
G-protein coupled acetylcholine receptor signaling pathway
gamma-aminobutyric acid signaling pathway
response to nutrient
cell proliferation
positive regulation of cell proliferation
intracellular signal transduction
regulation of calcium ion transport
metabolic process
G-protein coupled glutamate receptor signaling pathway
regulation of heart contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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